Synthesis and Characterization of New 1, 2,4,5-Tetraimidazole Derivatives Assisted -Ultrasound Irradiation and Study of their Preliminary Antibacterial Evaluation
Imidazole derivatives have a wide range of biological applications, including antifungal, antibacterial, antiviral, antioxidant, antiparasitic, and anticancer compounds. As a result, various imidazole derivatives have been produced and developed as potential drugs for the treatment of a variety of diseases in recent years. In this study, we describe the synthesis of new 1,2, 4, 5- tetrasubstituted imidazole’s (5a-d) with pyrazole-3-one moiety by using an ultrasonicate synthesis and compare with traditional methods, utilizing a simple synthetic process and an easy purification procedure. Ultrasonication is a simple, clean, rapid, efficient, and cost-effective process for synthesizing organic compounds that has become known as a tool for green chemistry. By using this method, chemical reactions can be sped up from hours to minutes. Traditional synthetic reaction processes require longer, more complex, and difficult equipment setup that increases costs and pollutes the environment. All of these newly synthesized derivatives were characterized by different spectroscopic techniques (FT-IR, NMR(1H,13C) and CHN analysis). The disk test diffusion method was used to evaluate the new imidazole derivatives as a potential antibacterial activity against the Gram-positive bacterium Staphylococcus aureus and the Gram-negative bacterium Escherichia coli and Klebsiella pneumonia. Among the compounds tested compounds 5b and 5c showed the highest antimicrobial activities at a concentration of 200μg/mL and 300μg/mL respectively.